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flow cytometric methods  (Clinical and Laboratory Standards Institute)

 
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    Clinical and Laboratory Standards Institute flow cytometric methods
    Flow Cytometric Methods, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometric methods/product/Clinical and Laboratory Standards Institute
    Average 90 stars, based on 1 article reviews
    flow cytometric methods - by Bioz Stars, 2026-03
    90/100 stars

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    Flow <t>cytometric</t> gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the <t>proliferation</t> gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.
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    Flow cytometric gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the proliferation gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.

    Journal: Journal of Clinical Pathology

    Article Title: Comparison of T-cell immune responses to SARS-CoV-2 spike (S) and nucleocapsid (N) protein using an in-house flow-cytometric assay in laboratory employees with and without previously confirmed COVID-19 in South Africa: nationwide cross-sectional study

    doi: 10.1136/jclinpath-2021-207556

    Figure Lengend Snippet: Flow cytometric gating strategies used to identify the response of T-cells to the N-protein and S-protein of SARS-CoV2. Gating was first set on singlet events (A), followed by gating on target populations, leucocytes (B) and then CD3 expressing T-cells (C). The stimulation of the T-cells were measured using intracellular—PE Ki-67 (D). Buffer was added to the negative control (unstimulated) in (D). The positive control in (E) was pokeweed mitogen. The stimulation of the T-cells can be seen in the proliferation gate (E). T-cell activation seen in the proliferation gate (F) when exposed to nucleocapsid (N) protein of SARS-CoV2. T-cell activation seen in the proliferation gate (G) when exposed to spike (S) protein of SARS-CoV2. SSC, side scatter.

    Article Snippet: A standardised, in-house flow cytometric lymphocyte proliferation test method currently in use at Ampath to detect T-cell responses to other recall antigens, for example, Varicella-Zoster-Virus (VZV), Candida albicans and Tetanus toxoid was modified for the purpose of this study.

    Techniques: Expressing, Negative Control, Positive Control, Activation Assay